The Drought-Stress Response of a Drought Resistant Impatiens Processed in PEG-6000 Solution in a Simulation Test

📝 Abstract

Premise of the Study: Impatiens is a commonly seen garden flower, renowned for its strong adaptability and long history of cultivation. However, seldom has any research touched on its physiological resistance mechanism. In this experiment, the impatiens is selected from those which experienced aerospace mutation and thereafter 12 years of cultivation and breeding. Therefore, it is superior to the non-mutagenized impatiens in terms of drought resistance and demonstrates tremendous differences from the normal impatiens in physiology, which intrigues scholars to search for the underlying reasons . Methods: By reference to Impatiens balsamina L,this experiment uses mutagenized impatiens seeds, processed by PEG-6000 in different solution concentration, to measure the germination rate of impatiens, its relative enzymatic activity and expression differences between gene SoS2 and gene RD29b in the drought lower reaches. Key results: Under simulated drought stress, there is no distinct difference between the mutagenized impatiens and the normal impatiens in terms of germination rate. But by measuring the root tillers and the length, the relative enzymatic activity, MDA, and the expression differences between gene SoS2 and gene RD29b in the drought lower reaches, it is verified that the mutagenized impatiens has more advantages than the normal impatiens, and it Can further cultivate become drought resistance varieties impatiens. Conclusions: In this experiment,which is a positive mutation, the mutagenized impatiens improves drought resistance through radiative mutation. The so obtained impatiens is more pleasing in sight in terms of color and shape and has higher application value in garden virescence.

💡 Analysis

Premise of the Study: Impatiens is a commonly seen garden flower, renowned for its strong adaptability and long history of cultivation. However, seldom has any research touched on its physiological resistance mechanism. In this experiment, the impatiens is selected from those which experienced aerospace mutation and thereafter 12 years of cultivation and breeding. Therefore, it is superior to the non-mutagenized impatiens in terms of drought resistance and demonstrates tremendous differences from the normal impatiens in physiology, which intrigues scholars to search for the underlying reasons . Methods: By reference to Impatiens balsamina L,this experiment uses mutagenized impatiens seeds, processed by PEG-6000 in different solution concentration, to measure the germination rate of impatiens, its relative enzymatic activity and expression differences between gene SoS2 and gene RD29b in the drought lower reaches. Key results: Under simulated drought stress, there is no distinct difference between the mutagenized impatiens and the normal impatiens in terms of germination rate. But by measuring the root tillers and the length, the relative enzymatic activity, MDA, and the expression differences between gene SoS2 and gene RD29b in the drought lower reaches, it is verified that the mutagenized impatiens has more advantages than the normal impatiens, and it Can further cultivate become drought resistance varieties impatiens. Conclusions: In this experiment,which is a positive mutation, the mutagenized impatiens improves drought resistance through radiative mutation. The so obtained impatiens is more pleasing in sight in terms of color and shape and has higher application value in garden virescence.

📄 Content

This work was funded by the 13th Five-Year Plan for Economic and Social Development of the People’s Republic of China—breeding research projects in sichuan province. 4Author for correspondence: Pan (scpyzls@163.com), phone: 86-13880886911 The Drought-Stress Response of a Drought Resistant Impatiens Processed in PEG-6000 Solution in a Simulation Test
Xue Lan Liao2,3 Guo Qin Wen3 Qing Lin Liu2 Xue Yang Li2 Meng Xi Wu2 and Yuan Zhi Pan2 ,4 Author Affiliations 2College of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan,China 3Key Laboratory of Southwest China Wildlife Resources Conservation (China West Normal University), Ministry of Education, Nanchong, Sichuan 637002, China Abstract: Premise of the Study: Impatiens is a commonly seen garden flower, renowned for its strong adaptability and long history of cultivation. However, seldom has any research touched on its physiological resistance mechanism. In this experiment, the impatiens is selected from those which experienced aerospace mutation and thereafter 12 years of cultivation and breeding. Therefore, it is superior to the non-mutagenized impatiens in terms of drought resistance and demonstrates tremendous differences from the normal impatiens in physiology, which intrigues scholars to search for the underlying reasons . Methods: By reference to Impatiens balsamina L,this experiment uses mutagenized impatiens seeds, processed by PEG-6000 in different solution concentration, to measure the germination rate of impatiens, its relative enzymatic activity and expression differences between gene SoS2 and gene RD29b in the drought lower reaches.
Key results: Under simulated drought stress , there is no distinct difference between the mutagenized impatiens and the normal impatiens in terms of germination rate. But by measuring the root tillers and the length, the relative enzymatic activity, MDA, and the expression differences between gene SoS2 and gene RD29b in the drought lower reaches, it is verified that the mutagenized impatiens has more advantages than the normal impatiens, and it Can further cultivate become drought resistance varieties impatiens. Conclusions: In this experiment,which is a positive mutation, the mutagenized impatiens improves drought resistance through radiative mutation. The so obtained impatiens is more pleasing in sight in terms of color and shape and has higher application value in garden virescence. Key words: mutagenized impatiens, germination of seed, adversity，PEG-6000 ， SOD POD, gene expression 2

 Impatiens is the biggest flower genus in Balsamine family.There are about 900 

species around the world, distributed mainly in tropical and subtropical mountain areas and sparsely in north temperate zone. The reported Impatiens in China has about 200 species, growing mainly in Southwest provinces. Impatiens, also known as Henna,Henna dye and peach flower, has a scientific name in Latin:Impatiens balsamina L. . Impatiens is an annual herbaceous flower widely planted in gardens for appreciation with long florescence, strong adaptability, and marvelous colors. It is so named due to the observation that its head,wing,tail and foot outstand like the phoenix bird and therefor plays some role in China’s flower culture. It is also named “camphire” and “daughter flower” due to the fact that it contains natural brick red pigment which can be used to paint fingernails. Because of the combination of ornamental and practical values, impatiens is much favored by Chinese intellectuals. As a result, it finds its frequent appearance in their literary works, which gives rise to the richly historied and highly connotative impatiens culture(CHAI Jihong,2012) In the past, plant biosystematists, domestic or foreign, conducted little research on Chinese Impatiens and the relative documents are in lack. A further study is needed on its constituents, growing mechanism and application value to fill the vacancy. At present, there is a large quantity of studies on the pharmacological constituents and operating mechanism of impatiens at home and abroad while little is on its physiological mechanism of resistance. Since the 60s of the last century, with the human exploration of space, opening the human using special environmental conditions of plant space mutagenesis, through breeding and many new varieties of crops and ornamental flowers. Research 3

shows that after space mutagenesis of plant material, shape, enzyme activity, flowering, etc will change(LIU Min et al.,2003; JIANG Yifan et al.,2007; CHEN Yu et al.,2013; ZHANG Yongfu et al.,2015). The impatiens balsamina L. used in this experiment is the aerospace mutated No. 2 impatiens in China West Normal University, descending from No.2 seed of the SP14th generation of the mutated impatiens balsamina L(TANG Zesheng,2007). The aerospace mutated No. 2 impatiens in China West Normal University appears re

This content is AI-processed based on ArXiv data.