CRISPR/Cas9 For Photoactivated Localization Microscopy (PALM)

📝 Original Info

• Title: CRISPR/Cas9 For Photoactivated Localization Microscopy (PALM)
• ArXiv ID: 1403.6738
• Date: 2014-03-27
• Authors: Researchers from original ArXiv paper

📝 Abstract

We demonstrate that endonuclease deficient Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated Cas9 protein (dCas9) fused to the photo-convertible fluorescence protein monomeric mEos3.1 (dCas9-mEos3) can be used to resolve sub-diffraction limited features of repetitive gene elements, thus providing a new route to investigate high-order chromatin organization at these sites.

💡 Deep Analysis

Deep Dive into CRISPR/Cas9 For Photoactivated Localization Microscopy (PALM).

We demonstrate that endonuclease deficient Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated Cas9 protein (dCas9) fused to the photo-convertible fluorescence protein monomeric mEos3.1 (dCas9-mEos3) can be used to resolve sub-diffraction limited features of repetitive gene elements, thus providing a new route to investigate high-order chromatin organization at these sites.

📄 Full Content

Reference